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iwadate @ yamaguchi-u.ac.jp
Faculty of Science
Yamaguchi University
Yamaguchi 753-8512, Japan


Lab Members
    Staff
      IWADATE Yoshiaki
      OKIMURA Chika

    Graduate and Undergraduate Students


Research Theme

We are interested in self-organization mechanisms in cell migrations, such as polarity in crawling cell migration and ciliary metachronal wave system and so on.

Crawling Cell Migration(keratocytes) Crawling Cell Migration(HL-60 cells)
taken by Okimura C taken by Okimura C

Ciliary Metachronal Wave (P. caudatum)
taken by Narematsu N


Approach and Techniques

Biophysical Approach.
Live cell imaging under microscopies; CLSM, TIRFM, Traction force Microscopy, ...
Mechatronics; lathe, CNC milling machine, ...
Electronics; OP Amp, CMOS, TTL, ...
Programing; LabVIEW, MATLAB, Quick BASIC, ...

CLSM
YOKOGAWA Spin Disk
Actin Filaments
in D. discoideum
under CLSM
Myosin II Filaments
in D. discoideum
under CLSM

Lathe Electronics Lab Made Cell
Stretching Device


Papers (2008-)

Okimura, C. and Iwadate, Y. (in press). Hybrid mechanosensing system to generate the polarity needed for migration in fish keratocytes. Cell Adhesion & Migration. doi: 10.1080/19336918.2016.1170268

Okimura, C., Ueda, K., Sakumura, Y. and Iwadate, Y. (in press). Fast-crawling cell types migrate to avoid the direction of periodic substratum stretching. Cell Adhesion & Migration. doi: 10.1080/19336918.2015.1129482

Sonoda, A., Okimura, C. and Iwadate, Y. (2016). Shape and area of keratocytes are related to the distribution and magnitude of their traction forces. Cell Structure and Function 41: 33-43. doi: 10.1247/csf.15008

Nakata, T., Okimura, C., Mizuno, T. and Iwadate, Y. (2016). The role of stress fibers in the shape determination mechanism of fish keratocytes. Biophysical Journal 110: 481-492. doi: 10.1016/j.bpj.2015.12.014

Narematsu, N., Quek, R., Chiam, K.-H. and Iwadate, Y. (2015). Ciliary metachronal wave propagation on the compliant surface of Paramecium cells. Cytoskeleton 72: 633-646. doi: 10.1002/cm.21266

Nakashima, H., Okimura, C. and Iwadate, Y. (2015). The molecular dynamics of crawling migration in microtubule-disrupted keratocytes. Biophysics and Physicobiology 12: 21-29. doi: 10.2142/biophysico.12.0_21

Tsugiyama, H., Okimura, C., Mizuno, T. and Iwadate, Y. (2013). Electroporation of adherent cells with low sample volumes on a microscope stage. Journal of Experimental Biology 216: 3591-3598. doi: 10.1242/jeb.089870

Iwadate, Y., Okimura, C., Sato, K., Nakashima, Y., Tsujioka, M. and Minami, K. (2013). Myosin-II-mediated directional migration of Dictyostelium cells in response to cyclic stretching of substratum. Biophysical Journal 104: 748-758. doi: 10.1016/j.bpj.2013.01.005

Uyeda, Q.P.T., Iwadate, Y., Umeki, N., Nagasaki, A. and Yumura, S. (2011). Stretching actin filaments within cells enhances their affinity for the myosin II motor domain. PLoS ONE 6: e26200. doi: 10.1016/j.bpj.2013.01.005

Iwadate, Y. and Yumura, S. (2009). Cyclic stretch of the substratum using a shape-memory alloy induces directional migration in Dictyostelium cells. Biotechniques 47: 757-767. doi: 10.2144/000113217

Pramanik, Md. K., Iijima, M., Iwadate, Y. and Yumura, S. (2009). PTEN is a mechanosensing signal transducer for myosin II localization in Dictyostelium cells. Genes to Cells 14: 821-834. doi: 10.1111/j.1365-2443.2009.01312.x

Iwadate, Y. and Nakaoka, Y. (2008). Calcium regulates independently ciliary beat and cell contraction in in Paramecium cells. Cell Calcium 44: 169-179. doi: 10.1016/j.ceca.2007.11.006

Iwadate, Y. and Yumura, S. (2008). Actin-based propulsive force and myosin II-based contractile force in a migrating Dictyostelium cells. Journal of Cell Science 121: 1314-1324. doi: 10.1242/jcs.021576

Iwadate, Y. and Yumura, S. (2008). Molecular dynamics and forces of a motile cell simultaneously visualized by TIRF and force microscopies. Biotechniques 44: 739-750. doi: 10.2144/000112752


Invited Talks (2008-)

Iwadate Y. and Hori M. Regulation of ciliary beats and metachronal ciliary wave propagation. Annual Meeting of Society of Aero Aqua Bio-Mechanisms. 3 Sep. 2015.

Iwadate Y. Cyclic stretching of substratum induces directional migration in fast-crawling cells. Symposium on “Modern Methods in Computing Biology”. Bioinformatics Institute, A*STAR, Singapore. 23-24. Mar. 2015.

Iwadate, Y. Traction forces and polarity for crawling cell migration. Innovative Cell Biology. Chiba Univ. Mar. 1, 2013.

Iwadate, Y. Polarity generation for cell migration by traction forces. The 9th Bio-Optics Conference. Yamaguchi Univ. Dec. 7-8, 2012.

Iwadate, Y. A possible mechanism of traction forces and directional migration in Dictyostelium cells. MBI Seminar and Workshop. National University of Singapore. Sep. 13-14, 2011.

Iwadate, Y. A possible mechanism of traction forces and directional migration in Dictyostelium cells. BMB2010. Kobe Portopia Hotel. Dec. 7-10, 2010.

Iwadate, Y. Measurement of traction force in amoeboid movement and dicision of migrating direction by mechano-sensing mechanism. The 2th Annual Meeting of the Japanese Society for Quantitative Biology. Osaka Univ. Jan. 10-11, 2010.

Iwadate, Y. and Yumura, S. Traction force and meshano-sensing system in Dictyostelium amoeba. Symposium on "Biology under Time-Space Hierarchy Project", The Kyushu University Research Superstar Program (SSP). Kyushu Univ. Feb. 21, 2008.


Grants (2008-)

Iwadate, Y. Research Project Number:15H01323. Ministry of Education, Science, Sports and Culture, Grant-in-Aid for Scientific Research. 2015-2016.

Iwadate, Y. Research Project Number:26103524. Ministry of Education, Science, Sports and Culture, Grant-in-Aid for Scientific Research. 2014-2015.

Iwadate, Y. Research Project Number:26650050. Ministry of Education, Science, Sports and Culture, Grant-in-Aid for Scientific Research. 2014-2016.

Iwadate, Y. Metachronal wave of ciliary beat on Paramecium cell surface. Ministry of Education, Science, Sports and Culture, Grant-in-Aid for Exploratory Research. 2013-2014.

Iwadate, Y. Polarity generation for cell migration by their own traction forces. Ministry of Education, Science, Sports and Culture, Grant-in-Aid for Exploratory Research. 2011-2012.

Iwadate, Y. Development of a visualization device of traction forces of migrating cells forin vitro histogenesis and regenerative medicine. A-STEP Project, JST. 2010.

Iwadate, Y. Traction forces and self-organized porality in Amoeboid movement. Basic Research Program in Yamaguchi Univ.. 2010.

Iwadate, Y. Understanding of clliary metachronal wave system. Ministry of Education, Science, Sports and Culture, Grant-in-Aid for Exploratory Research. 2010-2012.

Iwadate, Y. Development and application of a novel cell stretching device for in vitro histogenesis and regenerative medicine. Seeds Project, JST. 2009.

Iwadate, Y. Biological rhythm underlying expansion-contraction systems in amoeboid movements. The Dynamic Mechanism and Fundamental Technology for Biological System, PREST, JST. 2008-2011.



Faculty of Science, Yamaguchi University
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